Virtual Booth

Reprogramming technique for cancer stem cells presents complex challenges due to stringent cell culture procedures, cell behaviour variations, and ambiguous workflow, resulting in data inconsistency and compromised reproducibility. The OSSC-RePRO addresses these issues by integrating validated and optimised workflows into a structured, user-friendly framework. Essential steps for OSSC maintenance, reprogramming, and analysis are organised as definitive and standard instructions that minimises reliance on trial-and-error, hence enhances reproducibility and reliability across experiments. This technical guide advocates time and resource efficiency yet high reliability and reproducibility data towards advances of highly impactful discoveries in osteosarcoma metastasis research.