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The global demand for clean energy drives the search for sustainable solutions to reduce greenhouse gas emissions and dependence on fossil fuels. Algal bio-photovoltaic (BPV) systems offer an innovative approach by utilizing algae's photosynthetic properties to produce bioelectricity. This study examines the effect of irradiance levels (20–100 µmol photons on BPV performance. Results showed bioelectricity generation peaked at 80 µmol photons achieving a power density of 3.08 mW/m^2 before declining at 100 µmol photons due to photoinhibition. The findings highlight optimal light conditions for maximizing energy generation in algal BPV systems.