YC560: DESIGN OF SGRNAS FOR CRISPR-CAS9 TARGETING VARIANTS ASSOCIATED WITH TYPE 2 DIABETES
Ron Gabriel Jogno Tayug NHS
Type 2 diabetes mellitus (T2DM) is a growing global health concern with strong genetic contributions. This study employed in silico approaches to design and evaluate CRISPR-Cas9 single guide RNAs (sgRNAs) targeting T2DM-associated genes TCF7L2, SLC30A8, KCNJ11, CDKN2A/2B, and IRS1. Using Benchling and Cas-Offinder, sgRNAs were assessed for on-target efficiency and off-target specificity. Results showed variable sgRNA performance, with KCNJ11 exhibiting the highest on-target efficiency and TCF7L2 and IRS1 demonstrating superior specificity. Correlation and ANOVA analyses revealed no significant relationship or differences between scores. These findings provide a computational framework for precise gene-editing strategies in T2DM research.
